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Hel-N1 is a neural-specific RNA-binding protein which is highly conserved over evolution. The data presented here demonstrate alternative 5 splicing of Hel-N1 mRNA, characterized by the insertion of a novel 91-bp exon. The resultant isoform, Hel-N2, has a potentially expanded N-terminal region of 29 amino acids when compared to Hel-N1. Reverse transcription (RT)-PCR cloning data indicate that...
We have isolated and characterized the cDNA and genomic DNA encoding fibroblast growth factor receptor-4 of Xenopus laevis (XFGFR-4). The gene encompassing the total coding sequence spans about 10 kb, consists of 17 exons, and has an organization very similar to those of mammalian genes encoding FGFR-1 and -2, except that the XFGFR-4 gene does not contain an alternative exon for the third immunoglobulin-like...
Troponin T (TpnT), an essential component of the Ca 2+ -regulatory troponin complex, is involved in protein-protein interactions with other thin-filament proteins during muscle contraction in vertebrate striated muscle (VSM). The isoforms of TpnT are encoded by members of a multigene family which, by alternate splicing, produces a complex pattern of isoproteins in VSM. The functional...
The human thyroglobulin (hTg)-encoding gene is a single-copy gene of more than 300 kb and composed of at least 42 exons. After studying the 5 portion of the Tg cDNA (from nucleotides (nt) 1 to 842), we have previously suggested the existence of alternative splicing of the hTg pre-mRNA in human thyroid tissue. Here, we describe four alternative splicings in the central region of the hTg pre-mRNA...
We have isolated a complete cDNA encoding for the mouse erythrocyte protein 4.2 (P4.2). The entire P4.2 cDNA consists of 3465 nt with an open reading frame (ORF) of 691 amino acids. Northern blot analysis of mouse reticulocyte or spleen RNA using the P4.2 cDNA as a probe, detected a 3.5-kb message. The size of the mouse P4.2 cDNA or message that we obtained, appears to be different from those reported...
The mts1 gene is one of the genes specifically expressed in mouse metastatic tumors and tumor cell lines. In this paper, we present data on cloning and sequencing of two variants of human mts1 cDNAs (hu-mts1 and hu-mts1 (var)), as well as of the corresponding region in the human genome. Comparison of the genomic sequence with the sequence of the mts1 cDNAs demonstrates presence of two alternatively...
The complete gene encoding the human N-methyl-d-aspartate receptor subunit NR1 (NMDAR1) has been isolated on a single cosmid clone. The gene is composed of 21 exons distributed over a total length of about 31 kb. More than 24 kb were sequenced. Exons 4, 20 and 21 are identical in their amino-acid sequence to those exons that are subject to alternative splicing in rat, indicating that all eight...
The status of the p53 gene in SEWA-AS12-ADH (S-ADH) cells, a subline of the mouse sarcoma cell line SEWA, was examined. Immunoprecipitation with wild-type (wt) or mutant P53-specific monoclonal antibodies (mAb) showed that both wt and mutant P53 were produced. Sequence analysis of the p53 cDNA and genomic DNA revealed a single nucleotide (nt) substitution at a splice donor site at the beginning of...
We are characterizing the alternatively spliced human peptidylglycine α-amidating monooxygenase (hPAM)-encoding mRNA transcripts expressed by human cells. Reverse transcription coupled to the polymerase chain reaction (RT-PCR) has been used to identify four alternatively spliced variants that differ in the region joining the two catalytic domains. Two of the transcripts represent previously reported...
The PACAP-R gene, encoding the mouse pituitary adenylate cyclase-activating polypeptide receptor (PACAP-R), has been isolated and its structural organization has been determined. PACAP-R spans more than 50 kb and is divided into 18 exons. PACAP-R contains two alternative exons encoding the putative third intracellular loop, as found in the rat PACAP-R. The proximal promoter region is highly G +...
The existence of a third non-coding exon in the human neprilysin-encoding gene (h-NEP), positionally located as exon 3, has been demonstrated by reverse transcription of RNA from human kidney and lung, coupled with the polymerase chain reaction. Comparison of nucleotide sequences between h-NEP and the rat NEP (r-NEP) genes shows a high degree of sequence conservation within noncoding exons 1 and...
A polymorphism in the genes encoding α- and β-adducin (ADD) was described as being associated with blood-pressure variation in a genetically hypertensive strain of rats (MHS). ADD is a cytoskeletal heterodimeric protein which may be involved in cellular signal transduction and interacts with other membrane skeleton proteins which affect ion transport across the cell membrane. The cDNA encoding the...
Adducin (ADD) is a heterodimeric protein of the membrane skeleton with subunits of 103 (α) and 97 kDa (β). It promotes the assembly of the spectrin-actin network. We have previously shown that one point mutation in each of the α and β rat ADD-encoding genes is associated with blood pressure variation in an animal model for hypertension, the Milan hypertensive strain of rats, probably due to a change...
The entire gene encoding the rat acyl-CoA-binding protein (ACBP), and intron 1 of the mouse and human ACBP genes were sequenced and analyzed. A CpG island has been maintained in human ACBP which, in contrast to the rodent ACBP genes, is subject to alternative splicing. Analysis of the rat intron 2 and 3 sequences identified a number of potential alternative splice donor and splice acceptor sites...
A rat PCTAIRE-1 cDNA clone was isolated by immunoscreening of a PC12 cDNA library, followed by 5 RACE (rapid amplification of cDNA ends) to determine the 5 end. The rat PCTAIRE-1 cDNA sequence is 96% identical to mouse PCTAIRE-1 and contains an alternatively spliced exon of 131 bp near the 5 end. Although a mouse cDNA containing this exon has been reported, examination of several mouse cell...
A portion of the rat growth hormone receptor (GHR) gene was cloned by PCR. Restriction enzyme digestion and DNA sequence analyses revealed that an exon, exon 8A, encoding the carboxy terminus of the rat serum growth hormone binding protein (GHBP) was located between exons 7 and 8 of the rat GHR gene. Two tandem poly-adenylation (poly A) signals for the GHBP mRNA were found in the 3 -untranslated...
Using lipopolysaccharide (LPS)-stimulated equine peripheral blood mononuclear cell (PBMC) cDNA as a template, we performed polymerase chain reaction (PCR) amplification with equine interleukin-1β (IL-1β) specific primers. Electrophoresis of the PCR product on agarose gel revealed an additional smaller fragment that hybridized with an equine IL-1β cDNA probe. Sequencing of this fragment demonstrated...
We have isolated two novel cDNAs encoding the transforming growth factor-β (TGF-β) type II receptor (TGF-βIIR), termedTGF-βIIR α and TGF-βIIR β , from a human fetal liver library. They have unique nucleotide (nt) sequences, compared with the reported TGF-βIIR sequence, at the 5 end. Southern blot analysis using probes from each clone detected the specific genomic DNA fragments....
The gene that encodes the dorsal switch protein (DSP1) has been isolated from a Drosophila melanogaster cosmid library. It is organized into seven exons and six introns. The relative position of the introns within the region coding for the high mobility group (HMG) domains are identical to those of vertebrate HMG 12 genes. The close similarity between DSP1 and HMG12 genes strongly suggests that...
In a search for new protein tyrosine kinases (PTKs) in early hemopoietic cells, we have identified a sequence closely related to the Fibroblast Growth Factor Receptor (FGFR) family. A cDNA isolated from a mouse embryo library was 89% identical toFGFR-3 in both its coding and 3 untranslated regions. However, the region homologous to exons 5 to 9 of FGFR-3 was missing. In addition, the ORF was...
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